Volume 2 - 2012 - Issue 1

1. Construction and characterization of a genomic library of Colletotrichum horii from persimmon

Authors: Sun H, Zhang JZ, Zhang LL, Hyde KD

Recieved: 11 January 2012, Accepted: 11 January 2012, Published: 21 February 2012

A cosmid library from genomic DNA of Colletotrichum horii, the fungal pathogen causing persimmon anthracnose is constructed. We established a extraction method for obtaining 145-250 kb genomic DNA, which is suitable for a cosmid library construction. The cloning procedures were as follows: mycelia was obtained following conidial germination, smaller fragments were removed from purified DNA through capillary ligation of the DNA to the cosmid vector, and in vitro packaging into the lambda phage. The library presently consists of 4.78 × 104 independent clones with an average insert size of about 34 kb. This cosmid library is the first to be constructed for cloning the pathogenesis related genes of C. horii and for analyzing the molecular and genetic characteristics of this species.

Keywords: anthracnose – DNA purification – genomic DNA – pathogenesis


2. New fungi from Western Ghats. India

Authors: Hosagoudar VB and Sabeena A

Recieved: 05 January 2012, Accepted: 05 January 2012, Published: 12 March 2012

This paper gives an account of six taxa collected from the Western Ghats’ region of Peninsular India. Asterediella pittosporacearum, Meliola arkevermae, M. colubrinicola and Sarcinella embeliae are new species; Meliola kakkachiana var. poochiparensis is a new variety, and Palawaniella jasmini is reported for the first time from India. These taxa are described and illustrated.

Keywords: Asteridiella – Foliicolous fungi – Meliola – Palawaniella – Sarcinella


3. Pythium induced phenolic compounds in the resistance of Vitis vinifera to Botrytis cinerea

Authors: Bala K, Paul B

Recieved: 22 February 2012, Accepted: 28 February 2012, Published: 14 March 2012

Grey mold rot in grapevine is caused by a necrotrophic pathogen, Botrytis cinerea. There is an enormous loss in the quality and quantity of grapes in the Burgundian vineyards due to Botrytis infection. Our studies demonstrate that some species of Pythium are capable of inducing disease resistance in grapevine against the grey mold pathogen by accumulating phenolic compounds notably, ellagic, caffeic, gallic, caffeic, o-anisic, 3,4-dihydroxy benzoic and salicyclic acid. This is the first report on Pythium induced accumulation of phenolic compounds in the grapevine challenged by B. cinerea. Phenolic compounds produced in grapevine at different time periods after exposure to Pythium and B. cinerea were quantified and their significant increase in quantity was evaluated by statistical analyses.

Keywords: Botrytis cinerea – disease resistance – HPLC – oomycete – phenolic compounds – Pythium – Vitis vinifera


4. Colletotrichum gloeosporioides, the causal agent of citrus anthracnose in Guizhou Province

Authors: Jiang YL, Tan P, Zhou XY, Hou XL, Wang Y

Recieved: 27 February 2012, Accepted: 29 February 2012, Published: 19 March 2012

Ten Colletotrichum strains were isolated from leaf spots of Citrus in six different locations of Guizhou Province. Koch’s postulates were performed to prove these strains were the causal agents of the disease. Disease symptoms consist of small and irregular, yellow, brown or dark brown spots on the leaves. The fungal strains were identified as Colletotrichum gloeosporioides on the basis of morphology and ITS sequence data analyses.

Keywords: Coelomycetes – ITS – plant pathogen


5. Bipolaris cactivora causing fruit rot of dragon fruit imported from Vietnam

Authors: He PF, Ho H, Wu XX, Hou MS, He YQ

Recieved: 22 February 2012, Accepted: 28 February 2012, Published: 10 April 2012

In 2009, a new disease of imported dragon fruit (Hylocereus undatus) was discovered in a fruit market in Kunming, Yunnan Province of China. The symptoms included water-soaked, olive to black powdery spots to coalescing soft rot in the later stage. The pathogen of this disease was identified as Bipolaris cactivora and its pathogenicity was confirmed by satisfying Koch's postulates. This is the first report of B. cactivora causing fruit rot of dragon fruit imported from Vietnam. The need for vigilant quarantine measures is discussed.

Keywords: China – fungal pathogen – plant disease – Yunnan – Vietnam


6. Identity of powdery mildew on Senna in Mexico

Authors: Rodríguez-Alvarado G, Soto-Plancarte A, Fernández-Pavía SP, Betancourt-Resendes I, Braun U

Recieved: 18 March 2012, Accepted: 19 March 2012, Published: 10 April 2012

Two anamorphic powdery mildews (Erysiphales) on Senna spp., recently found in Mexico, have been morphologically and genetically examined, as well as subjected to pathogenicity tests. Powdery mildew on Senna occidentalis proved to belong to the Podosphaera xanthii complex (P. xanthii s. lat.), and another collection on S. septemtrionalis has been identified as Erysiphe (Pseudoidium) sp.

Keywords: Erysiphales – Erysiphe sp. – Podosphaera xanthii – Michoacán


7. Comprehensive check list of Cercosporoid fungi from Iran

Authors: Bakhshi M, Arzanlou M and Babai-Ahari A

Recieved: 05 March 2012, Accepted: 06 March 2012, Published: 10 April 2012

Little is known about the biodiversity of cercosporoid fungi in Iran. We provide a comprehensive literature-based checklist for 89 cercosporoid species known to occur on different plant species in Iran. The complete annotated list covers 29 Cercospora species, 24 Ramularia, 21 Passalora and 15 Pseudocercospora.

Keywords: hyphomycetes – taxonomy – mitosporic fungi


8. Tropical phytopathogens 2: Pseudocercospora fuligena

Authors: Phengsintham P, Chukeatirote E, McKenzie EHC, Hyde KD, Braun U

Recieved: 04 May 2012, Accepted: 07 May 2012, Published: 30 May 2012

Black leaf mold of tomato (Lycopersicon esculentum) is caused by Pseudocercospora fuligena, and the fungus and symptoms of the disease are illustrated and described. This cercosporoid disease causes leaf spots and spots on petioles, stems, and fruit peduncles (but not on the fruit itself). A detailed description of the lesion, morphological characters and partial sequence (273 bp) of the elongation factor 1-alpha (EF1a) gene of this species are provided.

Keywords: Anamorphic fungi – cercosporoid hyphomycetes – disease – morphology – tomato – South East Asia


9. Bipolaris spicifera isolates with unusual conidial germination pattern on sunflower from Iran

Authors: Arzanlou M, Khodaei S

Recieved: 01 May 2012, Accepted: 04 May 2012, Published: 03 June 2012

Bipolaris isolates with unusual conidial germination pattern were isolated from sunflower leaves in Northern Iran. Conidia germinated to form swollen germ tubes from both ends and swollen germ tubes underwent multiple branching soon after formation. The species was identified as Bipolaris spicifera based on phenotypic characteristics as well as sequence data from gpd and ITS-rDNA regions. Artificial inoculation of 4-week-old seedlings of sunflower under green house conditions induced chlorotic spots on sunflower leaves. This is first report on the occurrence of Bipolaris spicifera on sunflower in Iran. Our results on the unusual germination pattern of B. spicifera conidia, further stress the need for the implementation of molecular data for the identification of plant pathogenic Bipolaris species.

Keywords: gpd – ITS – leaf spot – sunflower – unusual conidium germination pattern


10. Cercospora senecionis-walkeri – a new leaf-spotting hyphomycete from Laos and Thailand

Authors: Phengsintham P, Chukeatirote E, McKenzie EHC, Hyde KD, Braun U

Recieved: 27 May 2012, Accepted: 30 May 2012, Published: 09 June 2012

A new leaf-spotting hyphomycete, Cercospora senecionis-walkeri, is described and illustrated based on material collected in Laos and Thailand. The new species is compared with other Cercospora species occurring on Senecio spp. in different parts of the world, and a key to Cercospora and Passalora species on hosts belonging to Senecio is provided.

Keywords: Asia– Cercospora – Cercosporoid fungus– new species


11. Screening of mycota associated with Aijung rice seed and their effects on seed germination and seedling vigour

Authors: Islam NF, Borthakur SK

Recieved: 02 May 2012, Accepted: 29 May 2012, Published: 20 June 2012

Experiments were conducted to evaluate the effect of some dominant seed borne fungi of Aijung rice variety on seed germination and seedling vigour. Twenty dominant fungi were found associated with Aijung rice seeds. Analysis of seed borne fungi by blotter method and agar plate method showed that species of Aspergillus, Fusarium, Alternaria and Curvularia are the dominant genera. Seed germination and seedling vigour tests were conducted using seed inoculation, soil inoculation and seed submergence method. Maximum reduction in seed germination and seedling vigour was caused by species of Fusarium in seed inoculation method, by species of Rhizopus and Fusarium in soil inoculation method and by species of Aspergillus in seed submergence method. In another experiment healthy rice seeds were soaked in 25, 50, 75 and 100% concentration of 7-, 14- and 21-day-old culture filtrates of the isolated seed borne fungi. Maximum reduction in seed germination was recorded from 21-day-old culture filtrates. The inhibitory effect on seed germination was found to decrease with increase in dilution of the filtrates.

Keywords: germination – incubation – seed inoculation – vigour index


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Plant Pathology & Quarantine Online publishes reviews, research articles, methodology papers, and taxonomic works in the field of plant pathology. The official journal language is English.


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